Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101881/GTX101881_43789_20200820_ICC_IF_R_w_23060100_538.webp "GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAD67 antibody
GTX101881
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat, Zebra Fish
TargetGAD1
Overview
- SupplierGeneTex
- Product NameGAD67 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.22 mg/ml
- ConjugateUnconjugated
- Gene ID2571
- Target nameGAD1
- Target descriptionglutamate decarboxylase 1
- Target synonymsCPSQ1, DEE89, GAD, SCP, glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, glutamate decarboxylase 1 (brain, 67kDa)
- HostRabbit
- IsotypeIgG
- Protein IDQ99259
- Protein NameGlutamate decarboxylase 1
- Scientific DescriptionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]
- ReactivityHuman, Monkey, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Borkowska P, Morys J, Zielinska A, et al. Effects of the Co-Overexpression of the BCL and BDNF Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton's-Jelly-Derived Mesenchymal Stem Cells. Biomedicines. 2023,11(6). doi: 10.3390/biomedicines11061751Read this paper
- Tsai CW, Tsai SJ, Pan YJ, et al. Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia. Neurotherapeutics. 2022,19(2):649-659. doi: 10.1007/s13311-022-01195-xRead this paper
- Ma X, Gao F, Chen Q, et al. ACE2 modulates glucose homeostasis through GABA signaling during metabolic stress. J Endocrinol. 2020,246(3):223-236. doi: 10.1530/JOE-19-0471Read this paper
- Ji MH, Lei L, Gao DP, et al. Neural network disturbance in the medial prefrontal cortex might contribute to cognitive impairments induced by neuroinflammation. Brain Behav Immun. 2020,89:133-144. doi: 10.1016/j.bbi.2020.06.001Read this paper
- Occelli F, Hasselmann F, Bourien J, et al. Age-related Changes in Auditory Cortex Without Detectable Peripheral Alterations: A Multi-level Study in Sprague-Dawley Rats. Neuroscience. 2019,404:184-204. doi: 10.1016/j.neuroscience.2019.02.002Read this paper
- Wang C, Yu B, Li M, et al. Two Groups of eGFP-Expressing Neurons with Distinct Characteristics in the Neocortex of GIN Mice. Neuroscience. 2019,404:268-281. doi: 10.1016/j.neuroscience.2019.01.026Read this paper
- Zhou Z, Zhang G, Li X, et al. Loss of phenotype of parvalbumin interneurons in rat prefrontal cortex is involved in antidepressant- and propsychotic-like behaviors following acute and repeated ketamine administration. Mol Neurobiol. 2015,51(2):808-19. doi: 10.1007/s12035-014-8798-2Read this paper
Datasheet
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