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ProductsBack/GAD67 antibody [3H2]
ICC/IF analysis of COS7 cells transiently transfected with GAD67 plasmid using GTX84470 GAD67 antibody [3H2].
ICC/IF analysis of COS7 cells transiently transfected with GAD67 plasmid using GTX84470 GAD67 antibody [3H2].
ICC/IF analysis of COS7 cells transiently transfected with GAD67 plasmid using GTX84470 GAD67 antibody [3H2].

GAD67 antibody [3H2]

GTX84470
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGAD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAD67 antibody [3H2] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H2
  • Concentration
    0.46 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2571
  • Target name
    GAD1
  • Target description
    glutamate decarboxylase 1
  • Target synonyms
    67 kDa glutamic acid decarboxylase; CPSQ1; DEE89; GAD; GAD-67; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); SCP
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ99259
  • Protein Name
    Glutamate decarboxylase 1
  • Scientific Description
    Catalyzes the production of GABA.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa.
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