More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GAD67 (GAD1) Mouse Monoclonal Antibody [Clone ID: OTI3G9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates [LY400290] (100ug) and [LC400290] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates [LY400290] (100ug) and [LC400290] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates [LY400290] (100ug) and [LC400290] (20ug) can be purchased separately from OriGene.

GAD67 (GAD1) Mouse Monoclonal Antibody [Clone ID: OTI3G9]

CF500329
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetGAD1
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    GAD67 (GAD1) Mouse Monoclonal Antibody [Clone ID: OTI3G9]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3G9
  • Gene ID2571
  • Target name
    GAD1
  • Target description
    glutamate decarboxylase 1
  • Target synonyms
    CPSQ1, DEE89, GAD, SCP, glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, glutamate decarboxylase 1 (brain, 67kDa)
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ99259
  • Protein Name
    Glutamate decarboxylase 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of mouse cells using GAD-65/67 Polyclonal Antibody
Product group Antibodies
Cusabio
GAD1/GAD2 AntibodyCSB-PA002622
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetGAD1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-GAD1 Antibody144-02938
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGAD1
  • SizePrice
Anti-GAD1 Antibody
Product group Antibodies
Antibodies.com
Anti-GAD1 AntibodyA96199
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of rat globus pallidus shows strong immunoreactivity in GABAergic neural fibers.
Product group Antibodies
Atlas Antibodies
Anti-GAD1 AntibodyAMAB91076
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAD1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GAD1 / GAD67 AntibodyLS-C766735
ApplicationsImmunoHistoChemistry
ReactivityMouse
TargetGAD1
  • SizePrice
Product group Antibodies
MedChem Express
GAD67 Antibody (YA1807, Rabbit)HY-P82062
TargetGAD1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-GAD1 (isoform GAD67)EB09109
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetGAD1
  • SizePrice
Figure 1. Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa.
Product group Antibodies
Boster Bio
Anti-GAD67/GAD1 Antibody Picoband(r)PB9183-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGAD1
  • SizePrice
Product group Antibodies
Assay Genie
GAD1/GAD2 AntibodyPACO06500
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetGAD1
  • SizePrice
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX101880 diluted at 1:500
Product group Antibodies
GeneTex
GAD67 antibody [N3C3]GTX101880
ApplicationsWestern Blot
ReactivityHuman
TargetGAD1
  • SizePrice