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ProductsBack/GADD45B antibody, Internal
IHC-P analysis of human prostate cancer tissue using GTX46244 GADD45B antibody at 2microg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC.
IHC-P analysis of human prostate cancer tissue using GTX46244 GADD45B antibody at 2microg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC.
IHC-P analysis of human prostate cancer tissue using GTX46244 GADD45B antibody at 2microg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC.

GADD45B antibody, Internal

GTX46244
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGADD45B
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Overview

  • Supplier
    GeneTex
  • Product Name
    GADD45B antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4616
  • Target name
    GADD45B
  • Target description
    growth arrest and DNA damage inducible beta
  • Target synonyms
    GADD45BETA; growth arrest and DNA damage-inducible protein GADD45 beta; MYD118; myeloid differentiation primary response protein MyD118; negative growth regulatory protein MyD118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75293
  • Protein Name
    Growth arrest and DNA damage-inducible protein GADD45 beta
  • Scientific Description
    This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD45B using anti-GADD45B antibody (A03508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45B antigen affinity purified polyclonal antibody (Catalog # A03508-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45B at approximately 18 kDa. The expected band size for GADD45B is at 18 kDa.
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