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GADD45B Polyclonal Antibody

CAC12901
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGADD45B
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Overview

  • Supplier
    Biomatik
  • Product Name
    GADD45B Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4616
  • Target name
    GADD45B
  • Target description
    growth arrest and DNA damage inducible beta
  • Target synonyms
    GADD45BETA; growth arrest and DNA damage-inducible protein GADD45 beta; MYD118; myeloid differentiation primary response protein MyD118; negative growth regulatory protein MyD118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75293
  • Protein Name
    Growth arrest and DNA damage-inducible protein GADD45 beta
  • Scientific Description
    The GADD45B Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD45B using anti-GADD45B antibody (A03508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45B antigen affinity purified polyclonal antibody (Catalog # A03508-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45B at approximately 18 kDa. The expected band size for GADD45B is at 18 kDa.
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