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ProductsBack/GAL4 (LGALS4) Rabbit Polyclonal Antibody
Western blot (WB) analysis of Galectin-4 antibody (Cat.-No.: AP20547PU-N) in extracts from COLO cells.
Western blot (WB) analysis of Galectin-4 antibody (Cat.-No.: AP20547PU-N) in extracts from COLO cells.
Western blot (WB) analysis of Galectin-4 antibody (Cat.-No.: AP20547PU-N) in extracts from COLO cells.

GAL4 (LGALS4) Rabbit Polyclonal Antibody

AP20547PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLGALS4
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GAL4 (LGALS4) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3960
  • Target name
    LGALS4
  • Target description
    galectin 4
  • Target synonyms
    GAL4, L36LBP, galectin-4, L-36 lactose-binding protein, antigen NY-CO-27, gal-4, lactose-binding lectin 4, lectin, galactoside-binding, soluble, 4
  • Host
    Rabbit
  • Protein IDP56470
  • Protein Name
    Galectin-4
  • Scientific Description
    GAL4 (LGALS4) rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS4 transfected 293T lysate 10% SDS PAGE GTX114527 diluted at 1:5000
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Figure 1. Western blot analysis of GAL4 using anti-GAL4 antibody (PB9952). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAL4 antigen affinity purified polyclonal antibody (Catalog # PB9952) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAL4 at approximately 39 kDa. The expected band size for GAL4 is at 36 kDa.
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