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ProductsBack/Galectin 3 antibody [5L36]

Galectin 3 antibody [5L36]

GTX53171
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetLgals3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Galectin 3 antibody [5L36]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5L36
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID16854
  • Target name
    Lgals3
  • Target description
    lectin, galactose binding, soluble 3
  • Target synonyms
    35 kDa lectin; carbohydrate-binding protein 35; CBP 35; ga; gal3; gal-3; galactose-specific lectin 3; galect; galectin-3; GBP; igE-binding protein; L-3; L-34; L-34 galactoside-binding lectin; laminin-binding protein; lectin L-29; Mac-; Mac-2; mac-2 antigen
  • Host
    Rat
  • Isotype
    IgG2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # PB9279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 29 kDa. The expected band size for Galectin 3/LGALS3 is at 29 kDa.
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