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ProductsBack/Goat anti-galectin-3 (mouse)

Goat anti-galectin-3 (mouse)

EB10775
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLgals3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-galectin-3 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID16854
  • Target name
    Lgals3
  • Target description
    lectin, galactose binding, soluble 3
  • Target synonyms
    GBP, L-34, Mac-2, gal3, galectin-3, 35 kDa lectin, CBP 35, L-34 galactoside-binding lectin, carbohydrate-binding protein 35, gal-3, galactose-specific lectin 3, igE-binding protein, laminin-binding protein, lectin L-29, mac-2 antigen
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_034835.1. GeneIDs all Nonhuman: 16854 (mouse);. Purification: Antigen affinity purified. Names and symbols: Lgals3; lectin, galactose binding, soluble 3; GBP; L-34; Mac-2; gal3; 35 kDa lectin; CBP 35; L-34 galactoside-binding lectin; OTTMUSP00000043688; OTTMUSP00000043689; OTTMUSP00000043690; carbohydrate-binding protein 35; gal-3; galactose-specific lectin 3;
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # PB9279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 29 kDa. The expected band size for Galectin 3/LGALS3 is at 29 kDa.
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