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Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Galectin 3 Polyclonal Antibody

BS-0721R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLGALS3
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Overview

  • Supplier
    Bioss
  • Product Name
    Galectin 3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3958
  • Target name
    LGALS3
  • Target description
    galectin 3
  • Target synonyms
    CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, galectin-3, 35 kDa lectin, IgE-binding protein, MAC-2 antigen, advanced glycation end-product receptor 3, carbohydrate-binding protein 35, epididymis secretory sperm binding protein, galactose-specific lectin 3, laminin-binding protein, lectin L-29, lectin, galactoside-binding, soluble, 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17931
  • Protein Name
    Galectin-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation. Zeng Y et al., 2018 Nov 6, Lipids Health Dis
    Read more

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Figure 1. Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody (A00621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # A00621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.
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