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Western blot All lanes: GALNT7 antibody at 0.1microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western blot All lanes: GALNT7 antibody at 0.1microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western blot All lanes: GALNT7 antibody at 0.1microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa

GALNT7 Antibody

CSB-PA773016LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGALNT7
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Overview

  • Supplier
    Cusabio
  • Product Name
    GALNT7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51809
  • Target name
    GALNT7
  • Target description
    polypeptide N-acetylgalactosaminyltransferase 7
  • Target synonyms
    GALNAC-T7, GalNAcT7, N-acetylgalactosaminyltransferase 7, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7), polypeptide GalNAc transferase 7, pp-GaNTase 7, protein-UDP acetylgalactosaminyltransferase 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86SF2
  • Protein Name
    N-acetylgalactosaminyltransferase 7
  • Scientific Description
    Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of GALNT7 using anti-GALNT7 antibody (A10021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT7 antigen affinity purified polyclonal antibody (Catalog # A10021-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT7 at approximately 75 kDa. The expected band size for GALNT7 is at 75 kDa.
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