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Anti-GALNT7 Antibody

107-10582
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGALNT7
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GALNT7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID51809
  • Target name
    GALNT7
  • Target description
    polypeptide N-acetylgalactosaminyltransferase 7
  • Target synonyms
    GALNAC-T7, GalNAcT7, N-acetylgalactosaminyltransferase 7, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7), polypeptide GalNAc transferase 7, pp-GaNTase 7, protein-UDP acetylgalactosaminyltransferase 7
  • Host
    Rabbit
  • Scientific Description
    GALNT7 antibody [N1C2], 1 mg/ml (100 ul)
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GALNT7 using anti-GALNT7 antibody (A10021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT7 antigen affinity purified polyclonal antibody (Catalog # A10021-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT7 at approximately 75 kDa. The expected band size for GALNT7 is at 75 kDa.
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