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ProductsBack/gamma Tubulin (TUBG1) Mouse Monoclonal Antibody [Clone ID: TU-30]
Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs togamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs togamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs togamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

gamma Tubulin (TUBG1) Mouse Monoclonal Antibody [Clone ID: TU-30]

AM03014PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot
DownloadMSDS
Product group Antibodies
TargetTUBG1
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    gamma Tubulin (TUBG1) Mouse Monoclonal Antibody [Clone ID: TU-30]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TU-30
  • Gene ID7283
  • Target name
    TUBG1
  • Target description
    tubulin gamma 1
  • Target synonyms
    CDCBM4; gamma-tubulin complex component 1; GCP-1; TUBG; TUBGCP1; tubulin gamma-1 chain; tubulin, gamma polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP23258
  • Protein Name
    Tubulin gamma-1 chain
  • Scientific Description
    gamma Tubulin (TUBG1) mouse monoclonal antibody, clone TU-30, Aff - Purified
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TUBG1/2 using anti-TUBG1/2 antibody (A06313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1/2 antigen affinity purified polyclonal antibody (Catalog # A06313-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBG1/2 at approximately 51 kDa. The expected band size for TUBG1/2 is at 51 kDa.
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