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Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

gamma Tubulin antibody

GTX113286
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetTUBG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    gamma Tubulin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.24 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7283
  • Target name
    TUBG1
  • Target description
    tubulin gamma 1
  • Target synonyms
    CDCBM4, GCP-1, TUBG, TUBGCP1, tubulin gamma-1 chain, gamma-tubulin complex component 1, tubulin, gamma polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li N, Xu Y, Chen H, et al. Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities. EBioMedicine. 2024,99:104940. doi: 10.1016/j.ebiom.2023.104940
    Read this paper
  • Dranow DB, Le Pabic P, Schilling TF. The non-canonical Wnt receptor Ror2 is required for cartilage cell polarity and morphogenesis of the craniofacial skeleton in zebrafish. Development. 2023,150(8). doi: 10.1242/dev.201273
    Read this paper
  • Ying Z, Wang K, Wu J, et al. CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72. BMC Biol. 2022,20(1):240. doi: 10.1186/s12915-022-01437-6
    Read this paper
  • Mahabaleshwar H, Asharani PV, Loo TY, et al. Slit-Robo signalling establishes a Sphingosine-1-phosphate gradient to polarise fin mesenchyme. EMBO Rep. 2022,23(8):e54464. doi: 10.15252/embr.202154464
    Read this paper
  • Gupta A, Thirugnanam K, Thamilarasan M, et al. Cilia proteins are biomarkers of altered flow in the vasculature. JCI Insight. 2022,7(6). doi: 10.1172/jci.insight.151813
    Read this paper
  • Lee WJ, Tu SH, Cheng TC, et al. Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues. Molecules. 2021,26(21). doi: 10.3390/molecules26216548
    Read this paper
  • Terhune EA, Cuevas MT, Monley AM, et al. Mutations in KIF7 implicated in idiopathic scoliosis in humans and axial curvatures in zebrafish. Hum Mutat. 2021,42(4):392-407. doi: 10.1002/humu.24162
    Read this paper
  • Lagos-Cabré R, Ivanova A, Taylor CW. Ca(2+) Release by IP(3) Receptors Is Required to Orient the Mitotic Spindle. Cell Rep. 2020,33(11):108483. doi: 10.1016/j.celrep.2020.108483
    Read this paper
  • Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. Nat Commun. 2019,10(1):5681. doi: 10.1038/s41467-019-13475-w
    Read this paper
  • Honjo Y, Ichinohe T. Cellular responses to ionizing radiation change quickly over time during early development in zebrafish. Cell Biol Int. 2019,43(5):516-527. doi: 10.1002/cbin.11117
    Read this paper

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Figure 1. Western blot analysis of TUBG1/2 using anti-TUBG1/2 antibody (A06313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1/2 antigen affinity purified polyclonal antibody (Catalog # A06313-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBG1/2 at approximately 51 kDa. The expected band size for TUBG1/2 is at 51 kDa.
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