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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GAP-43 Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GAP-43 Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GAP-43 Monoclonal Antibody.

GAP43 Monoclonal Antibody

CSB-MA080173
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGAP43
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAP43 Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2596
  • Target name
    GAP43
  • Target description
    growth associated protein 43
  • Target synonyms
    axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; nerve growth-related peptide GAP43; neural phosphoprotein B-50; neuromodulin; neuron growth-associated protein 43; PP46; protein F1
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP17677
  • Protein Name
    Neuromodulin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAP43 using anti-GAP43 antibody (A01868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # A01868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 38-43KD. The expected band size for GAP43 is at 38-43KD.
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