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ProductsBack/GAPDH Polyclonal Antibody
293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

GAPDH Polyclonal Antibody

BS-10900R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAPDH
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Overview

  • Supplier
    Bioss
  • Product Name
    GAPDH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Efficacy evaluation and mechanism study on inhibition of breast cancer cell growth by multimodal targeted nanobubbles carrying AMD070 and ICG. Shen D et al., 2020 Mar 27, Nanotechnology
    Read this paper
  • Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Wang T et al., 2020 Apr 15, Life Sci
    Read this paper
  • Sodium tanshinone IIA sulfonate protects against Abeta1-42-induced cellular toxicity by modulating Abeta-degrading enzymes in HT22 cells. Liu XQ et al., 2020 May 15, Int J Biol Macromol
    Read this paper
  • The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Wang T et al., 2020 Mar, Mol Med Rep
    Read this paper
  • Gypenoside A protects ischemia/reperfusion injuries by suppressing miR-143-3p level via the activation of AMPK/Foxo1 pathway. Chang L et al., 2020 May, Biofactors
    Read this paper
  • Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Yu B et al., 2020 Feb, Exp Mol Pathol
    Read this paper
  • The lysosomal membrane protein LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy. Liu X et al., 2020 Mar, FEBS Lett
    Read this paper
  • Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Liu X et al., 2019 Sep 15, Life Sci
    Read this paper
  • Inhibition of microRNA-143-3p attenuates myocardial hypertrophy by inhibiting inflammatory response. Yu B et al., 2018 Nov, Cell Biol Int
    Read this paper
  • Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1alpha. Jia Z et al., 2018 Sep, J Cell Mol Med
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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