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293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

GAPDH Polyclonal Antibody

BS-10900R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAPDH
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Overview

  • Supplier
    Bioss
  • Product Name
    GAPDH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shen D, Zhu L, Liu Y, et al. Efficacy evaluation and mechanism study on inhibition of breast cancer cell growth by multimodal targeted nanobubbles carrying AMD070 and ICG. Nanotechnology. 2020,31(24):245102. doi: 10.1088/1361-6528/ab7e73
    Read this paper
  • Wang T, Xie YQ, Miao GX, et al. Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Life Sci. 2020,247:117421. doi: 10.1016/j.lfs.2020.117421
    Read this paper
  • Liu XQ, Deng YX, Dai Z, et al. Sodium tanshinone IIA sulfonate protects against Aβ(1-42)-induced cellular toxicity by modulating Aβ-degrading enzymes in HT22 cells. Int J Biol Macromol. 2020,151:47-55. doi: 10.1016/j.ijbiomac.2020.02.040
    Read this paper
  • Wang T, Sun X, Cui H, et al. The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Mol Med Rep. 2020,21(3):1097-1106. doi: 10.3892/mmr.2020.10934
    Read this paper
  • Chang L, Shi R, Wang X, et al. Gypenoside A protects ischemia/reperfusion injuries by suppressing miR-143-3p level via the activation of AMPK/Foxo1 pathway. Biofactors. 2020,46(3):432-440. doi: 10.1002/biof.1601
    Read this paper
  • Yu B, Yu M, Zhang H, et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2020,112:104346. doi: 10.1016/j.yexmp.2019.104346
    Read this paper
  • Liu X, Liao X, Rao X, et al. The lysosomal membrane protein LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy. FEBS Lett. 2020,594(5):823-840. doi: 10.1002/1873-3468.13663
    Read this paper
  • Liu X, Tu B, Jiang X, et al. Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Life Sci. 2019,233:116741. doi: 10.1016/j.lfs.2019.116741
    Read this paper
  • Yu B, Zhao Y, Zhang H, et al. Inhibition of microRNA-143-3p attenuates myocardial hypertrophy by inhibiting inflammatory response. Cell Biol Int. 2018,42(11):1584-1593. doi: 10.1002/cbin.11053
    Read this paper
  • Jia Z, Wang X, et al. Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α. J Cell Mol Med. 2018,22(9):4507-4521. doi: 10.1111/jcmm.13763
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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