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GAPDH antibody

GTX100118
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBacteria, Bovine, Canine, C. Elegans, Chicken, Drosophila, Fish, Hamster, Human, Insect, Mammals, Monkey, Mouse, Plant, Porcine, Rabbit, Rat, Zebra Fish, Other Species
TargetGAPDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAPDH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.08 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
  • Reactivity
    Bacteria, Bovine, Canine, C. Elegans, Chicken, Drosophila, Fish, Hamster, Human, Insect, Mammals, Monkey, Mouse, Plant, Porcine, Rabbit, Rat, Zebra Fish, Other Species
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yuan M, Mahmud I, Katsushima K, et al. miRNA-211 maintains metabolic homeostasis in medulloblastoma through its target gene long-chain acyl-CoA synthetase 4. Acta Neuropathol Commun. 2023,11(1):203. doi: 10.1186/s40478-023-01684-w
    Read this paper
  • Wang YY, Cheng KH, Hung AC, et al. Differential impact of cytoplasmic vs. nuclear RAD51 expression on breast cancer progression and patient prognosis. Int J Oncol. 2024,64(2):pii: 12. doi: 10.3892/ijo.2023.5600.
    Read this paper
  • Li X, Shi R, Yan L, et al. Natural product rhynchophylline prevents stress-induced hair graying by preserving melanocyte stem cells via the β2 adrenergic pathway suppression. Nat Prod Bioprospect. 2023,13(1):54. doi: 10.1007/s13659-023-00421-z
    Read this paper
  • Tateishi K, Miyake Y, Nakamura T, et al. Genetic alterations that deregulate RB and PDGFRA signaling pathways drive tumor progression in IDH2-mutant astrocytoma. Acta Neuropathol Commun. 2023,11(1):186. doi: 10.1186/s40478-023-01683-x
    Read this paper
  • Chen S, Qin Y, Ye X, et al. Neurotoxicity of the Cu(OH)(2) Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish. Environ Sci Technol. 2023,57(48):19407-19418. doi: 10.1021/acs.est.3c06284
    Read this paper
  • Wang BY, Shen HT, Lee YL, et al. Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression. Am J Cancer Res. 2023,13(10):4721-4733.
    Read this paper
  • Chen H, Fan L, Guo Q, et al. DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicity. Res Sq. 2023,:pii: rs.3.rs-3454358. doi: 10.21203/rs.3.rs-3454358/v1.
    Read this paper
  • Liao WT, Chiang YJ, Yang-Yen HF, et al. CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling. J Biol Chem. 2023,299(12):105455. doi: 10.1016/j.jbc.2023.105455
    Read this paper
  • Wang CM, Wu CY, Lin CE, et al. Forgotten memory storage and retrieval in Drosophila. Nat Commun. 2023,14(1):7153. doi: 10.1038/s41467-023-42753-x
    Read this paper
  • Cheng HC, Huang PH, Lai FJ, et al. Loss of fragile WWOX gene leads to senescence escape and genome instability. Cell Mol Life Sci. 2023,80(11):338. doi: 10.1007/s00018-023-04950-1
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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