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GAPDH antibody

GTX100118
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAPDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAPDH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.08 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yuan M, Mahmud I, Katsushima K, et al. miRNA-211 maintains metabolic homeostasis in medulloblastoma through its target gene long-chain acyl-CoA synthetase 4. Acta Neuropathol Commun. 2023,11(1):203. doi: 10.1186/s40478-023-01684-w
    Read this paper
  • Wang YY, Cheng KH, Hung AC, et al. Differential impact of cytoplasmic vs. nuclear RAD51 expression on breast cancer progression and patient prognosis. Int J Oncol. 2024,64(2):pii: 12. doi: 10.3892/ijo.2023.5600.
    Read this paper
  • Li X, Shi R, Yan L, et al. Natural product rhynchophylline prevents stress-induced hair graying by preserving melanocyte stem cells via the β2 adrenergic pathway suppression. Nat Prod Bioprospect. 2023,13(1):54. doi: 10.1007/s13659-023-00421-z
    Read this paper
  • Tateishi K, Miyake Y, Nakamura T, et al. Genetic alterations that deregulate RB and PDGFRA signaling pathways drive tumor progression in IDH2-mutant astrocytoma. Acta Neuropathol Commun. 2023,11(1):186. doi: 10.1186/s40478-023-01683-x
    Read this paper
  • Chen S, Qin Y, Ye X, et al. Neurotoxicity of the Cu(OH)(2) Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish. Environ Sci Technol. 2023,57(48):19407-19418. doi: 10.1021/acs.est.3c06284
    Read this paper
  • Wang BY, Shen HT, Lee YL, et al. Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression. Am J Cancer Res. 2023,13(10):4721-4733.
    Read this paper
  • Chen H, Fan L, Guo Q, et al. DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicity. Res Sq. 2023,:pii: rs.3.rs-3454358. doi: 10.21203/rs.3.rs-3454358/v1.
    Read this paper
  • Liao WT, Chiang YJ, Yang-Yen HF, et al. CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling. J Biol Chem. 2023,299(12):105455. doi: 10.1016/j.jbc.2023.105455
    Read this paper
  • Wang CM, Wu CY, Lin CE, et al. Forgotten memory storage and retrieval in Drosophila. Nat Commun. 2023,14(1):7153. doi: 10.1038/s41467-023-42753-x
    Read this paper
  • Cheng HC, Huang PH, Lai FJ, et al. Loss of fragile WWOX gene leads to senescence escape and genome instability. Cell Mol Life Sci. 2023,80(11):338. doi: 10.1007/s00018-023-04950-1
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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