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ProductsBack/GAREM / FAM59A Antibody (FITC)

GAREM / FAM59A Antibody (FITC)

LS-C397261
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetGAREM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GAREM / FAM59A Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID64762
  • Target name
    GAREM1
  • Target description
    GRB2 associated regulator of MAPK1 subtype 1
  • Target synonyms
    C18orf11, FAM59A, GAREM, Gm944, GRB2-associated and regulator of MAPK protein 1, GRB2 associated regulator of MAPK1 1, GRB2 associated, regulator of MAPK1, GRB2-associated and regulator of MAPK1, Grb2-associated and regulator of Erk/MAPK, family with sequence similarity 59, member A, protein FAM59A
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAREM1 using anti-GAREM1 antibody (A12630-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAREM1 antigen affinity purified polyclonal antibody (Catalog # A12630-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAREM1 at approximately 97 kDa. The expected band size for GAREM1 is at 97 kDa.
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