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WB analysis of mouse stomach tissue lysate using GTX57235 Gastrin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse stomach tissue lysate using GTX57235 Gastrin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse stomach tissue lysate using GTX57235 Gastrin antibody. Dilution : 1:1000 Loading : 25microg per lane

Gastrin antibody

GTX57235
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetGAST
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Overview

  • Supplier
    GeneTex
  • Product Name
    Gastrin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2520
  • Target name
    GAST
  • Target description
    gastrin
  • Target synonyms
    GAS; gastrin; preprogastrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01350
  • Protein Name
    Gastrin
  • Scientific Description
    Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Gastrin/GAST using anti-Gastrin/GAST antibody (A03612-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastrin/GAST antigen affinity purified polyclonal antibody (Catalog # A03612-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastrin/GAST at approximately 20 kDa. The expected band size for Gastrin/GAST is at 11 kDa.
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