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Western Blot analysis of HeLa cells using GATA-4 Polyclonal Antibody
Western Blot analysis of HeLa cells using GATA-4 Polyclonal Antibody
Western Blot analysis of HeLa cells using GATA-4 Polyclonal Antibody

GATA4 Antibody

CSB-PA002646
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGATA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    GATA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2626
  • Target name
    GATA4
  • Target description
    GATA binding protein 4
  • Target synonyms
    ASD2; GATA-binding factor 4; TACHD; TOF; transcription factor GATA-4; VSD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43694
  • Protein Name
    Transcription factor GATA-4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
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