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Formalin-fixed and paraffin embedded human embryonic cells labeled with Anti-GATA-4, Unconjugated (bs-1778R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human embryonic cells labeled with Anti-GATA-4, Unconjugated (bs-1778R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human embryonic cells labeled with Anti-GATA-4, Unconjugated (bs-1778R) followed by conjugation to the secondary antibody and DAB staining

GATA4 Polyclonal Antibody

BS-1778R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
TargetGATA4
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Overview

  • Supplier
    Bioss
  • Product Name
    GATA4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2626
  • Target name
    GATA4
  • Target description
    GATA binding protein 4
  • Target synonyms
    ASD2, TACHD, TOF, VSD1, transcription factor GATA-4, GATA-binding factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43694
  • Protein Name
    Transcription factor GATA-4
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wu P, Sun Z, Lv X, et al. Fluoride Induces Autoimmune Orchitis Involved with Enhanced IL-17A Secretion in Mice Testis. J Agric Food Chem. 2019,67(48):13333-13343. doi: 10.1021/acs.jafc.9b05789
    Read this paper
  • Kim CW, Go RE, Ko EB, et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2020,35(1):66-77. doi: 10.1002/tox.22843
    Read this paper
  • Yang Z, Shi J, Guo Z, et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere. 2019,231:151-160. doi: 10.1016/j.chemosphere.2019.05.129
    Read this paper
  • Gao LR, Zhang NK, Ding QA, et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. Cell Transplant. 2013,22(10):1883-900. doi: 10.3727/096368912X662444
    Read this paper

Datasheet

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Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
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