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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, Rat stomach tissue All lanes: GATAD2A antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 66 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, Rat stomach tissue All lanes: GATAD2A antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 66 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, Rat stomach tissue All lanes: GATAD2A antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 66 kDa Observed band size: 69 kDa

GATAD2A Antibody

CSB-PA803156LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetGATAD2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    GATAD2A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54815
  • Target name
    GATAD2A
  • Target description
    GATA zinc finger domain containing 2A
  • Target synonyms
    p66alpha, transcriptional repressor p66-alpha, GATA zinc finger domain-containing protein 2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YP4
  • Protein Name
    Transcriptional repressor p66-alpha
  • Scientific Description
    Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of p66 alpha using anti-p66 alpha antibody (M09194). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p66 alpha antigen affinity purified monoclonal antibody (Catalog # M09194) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p66 alpha at approximately 68 kDa. The expected band size for p66 alpha is at 68 kDa.
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Immunohistochemical staining of human colon, placenta, skeletal muscle and testis using Anti-GATAD2A antibody HPA006759 (A) shows similar protein distribution across tissues to independent antibody HPA024373 (B).
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