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GBA2 Antibody

LS-C747904
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetGBA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GBA2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 72kDa/98kDa/104kDa, while the observed MW by Western blot was 105kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 72kDa/98kDa/104kDa, while the observed MW by Western blot was 105kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57704
  • Target name
    GBA2
  • Target description
    glucosylceramidase beta 2
  • Target synonyms
    AD035, NLGase, SPG46, non-lysosomal glucosylceramidase, beta-glucocerebrosidase 2, bile acid beta-glucosidase GBA2, bile acid glucosyl transferase GBA2, cholesterol glucosyltransferase GBA2, cholesteryl-beta-glucosidase GBA2, glucocerebrosidase 2, glucosidase, beta (bile acid) 2, glucosylceramidase 2, non-lysosomal cholesterol glycosyltransferase, non-lysosomal galactosylceramidase, non-lysosomal glycosylceramidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GBA2 using anti-GBA2 antibody (A08047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA2 antigen affinity purified polyclonal antibody (Catalog # A08047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA2 at approximately 105 kDa. The expected band size for GBA2 is at 105 kDa.
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