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Western blot analysis of extracts from HuvEc cells, using GBA3 antibody.
Western blot analysis of extracts from HuvEc cells, using GBA3 antibody.
Western blot analysis of extracts from HuvEc cells, using GBA3 antibody.

GBA3 Antibody

CSB-PA913325
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGBA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GBA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57733
  • Target name
    GBA3
  • Target description
    glucosylceramidase beta 3 (gene/pseudogene)
  • Target synonyms
    CBG, CBGL1, GLUC, KLRP, cytosolic beta-glucosidase, cytosolic GCase, cytosolic beta-glucosidase-like protein 1, cytosolic galactosylceramidase, cytosolic glucosylceramidase, cytosolic glycosylceramidase, glucosidase beta acid 3, glucosidase, beta, acid 3 (cytosolic), klotho-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H227
  • Protein Name
    Cytosolic beta-glucosidase
  • Scientific Description
    Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. Yahata K., J. Mol. Med. 78:389-394(2000). de Graaf M., Biochem. J. 356:907-910(2001). Berrin J.-G., Eur. J. Biochem. 269:249-258(2002)
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western Blot analysis of HuvEc cells using CBG Polyclonal Antibody
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Figure 1. Western blot analysis of GBA3 using anti-GBA3 antibody (A08549-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA3 antigen affinity purified polyclonal antibody (Catalog # A08549-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA3 at approximately 50 kDa. The expected band size for GBA3 is at 54 kDa.
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