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GBP1 Rabbit mAb

A0570
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetGBP1
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Overview

  • Supplier
    ABclonal
  • Product Name
    GBP1 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2633
  • Target name
    GBP1
  • Target description
    guanylate binding protein 1
  • Target synonyms
    hGBP1, guanylate-binding protein 1, GBP-1, GTP-binding protein 1, guanine nucleotide-binding protein 1, guanylate binding protein 1, interferon-inducible, 67kDa, huGBP-1, interferon-induced guanylate-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32455
  • Protein Name
    Guanylate-binding protein 1
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GBP1 using anti-GBP1 antibody (A03067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysayes. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBP1 antigen affinity purified polyclonal antibody (Catalog # A03067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBP1 at approximately 68 kDa. The expected band size for GBP1 is at 68 kDa.
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