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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GCHFR antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GCHFR antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GCHFR antibody.

GCHFR Antibody

CSB-PA109145
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGCHFR
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Overview

  • Supplier
    Cusabio
  • Product Name
    GCHFR Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2644
  • Target name
    GCHFR
  • Target description
    GTP cyclohydrolase I feedback regulator
  • Target synonyms
    GFRP, HsT16933, P35, GTP cyclohydrolase 1 feedback regulatory protein, GTP cyclohydrolase I feedback regulatory protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30047
  • Protein Name
    GTP cyclohydrolase 1 feedback regulatory protein
  • Scientific Description
    Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine. Bonner T.I., Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases. The MGC Project Team, Genome Res. 14:2121-2127(2004). Hochstrasser D.F., Electrophoresis 13:992-1001(1992).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of GCHFR using anti-GCHFR antibody (A08185-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCHFR antigen affinity purified polyclonal antibody (Catalog # A08185-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCHFR at approximately 12KD. The expected band size for GCHFR is at 12KD.
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