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GCLM antibody

MBS5310006
MyBioSource
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Product group Antibodies
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0.05 ml
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Overview

  • Supplier
    MyBioSource
  • Product Name
    GCLM antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Clone ID
    AT2D12
  • Concentration
    1 mg/ml
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.
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