
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM(GTX111858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GCLM antibody [N1N3]
GTX111858
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGCLM
Overview
- SupplierGeneTex
- Product NameGCLM antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2730
- Target nameGCLM
- Target descriptionglutamate-cysteine ligase modifier subunit
- Target synonymsGLCLR, glutamate--cysteine ligase regulatory subunit, GCS light chain, GSC light chain, gamma-ECS regulatory subunit, gamma-glutamylcysteine synthetase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD), glutamate-cysteine ligase modifier subunit delta2 alternative splicing, glutamate-cysteine ligase regulatory protein
- HostRabbit
- IsotypeIgG
- Protein IDP48507
- Protein NameGlutamate--cysteine ligase regulatory subunit
- Scientific DescriptionGlutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3] (GTX111858) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3] (GTX111858) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111858/GTX111858_40114_20160728_WB_shRNA_watermark_w_23060500_195.webp)








![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [HL3517] (GTX641414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641414/GTX641414_T-45614_20241206_WB_24121100_276.webp)