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GCLM Antibody (YA1407, Rabbit)

HY-P81662
MedChem Express
TargetGCLM
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GCLM Antibody (YA1407, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2730
  • Target name
    GCLM
  • Target description
    glutamate-cysteine ligase modifier subunit
  • Target synonyms
    GLCLR, glutamate--cysteine ligase regulatory subunit, GCS light chain, GSC light chain, gamma-ECS regulatory subunit, gamma-glutamylcysteine synthetase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD), glutamate-cysteine ligase modifier subunit delta2 alternative splicing, glutamate-cysteine ligase regulatory protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    GCLM Antibody (YA1407) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1407), targeting GCLM, with a predicted molecular weight of 31 kDa (observed band size: 31 kDa). GCLM Antibody (YA1407) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.
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