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ProductsBack/GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]
Western blot using GCLM / GLCLR antibody no. : The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot using GCLM / GLCLR antibody no. : The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot using GCLM / GLCLR antibody no. : The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]

AM50016PU-S
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGCLM
Price on request
50 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT2D12
  • Gene ID2730
  • Target name
    GCLM
  • Target description
    glutamate-cysteine ligase modifier subunit
  • Target synonyms
    GLCLR, glutamate--cysteine ligase regulatory subunit, GCS light chain, GSC light chain, gamma-ECS regulatory subunit, gamma-glutamylcysteine synthetase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD), glutamate-cysteine ligase modifier subunit delta2 alternative splicing, glutamate-cysteine ligase regulatory protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP48507
  • Protein Name
    Glutamate--cysteine ligase regulatory subunit
  • Scientific Description
    GCLM mouse monoclonal antibody, clone AT2D12, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.
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