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IHC-P analysis of mouse small intestine tissue using GTX03461 GDF8 / Myostatin antibody. Dilution : 1:200
IHC-P analysis of mouse small intestine tissue using GTX03461 GDF8 / Myostatin antibody. Dilution : 1:200
IHC-P analysis of mouse small intestine tissue using GTX03461 GDF8 / Myostatin antibody. Dilution : 1:200

GDF8 / Myostatin antibody

GTX03461
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMSTN
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Overview

  • Supplier
    GeneTex
  • Product Name
    GDF8 / Myostatin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. IHC-P: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2660
  • Target name
    MSTN
  • Target description
    myostatin
  • Target synonyms
    GDF8, MSLHP, growth/differentiation factor 8, myostatin-b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14793
  • Protein Name
    Growth/differentiation factor 8
  • Scientific Description
    This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GDF8/Myostatin/MSTN using anti-GDF8/Myostatin/MSTN antibody (A00926-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDF8/Myostatin/MSTN antigen affinity purified polyclonal antibody (Catalog # A00926-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDF8/Myostatin/MSTN at approximately 50KD. The expected band size for GDF8/Myostatin/MSTN is at 50KD.
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