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ProductsBack/GEF H1 Antibody (YA1877)

GEF H1 Antibody (YA1877)

HY-P82132
MedChem Express
TargetARHGEF2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GEF H1 Antibody (YA1877)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9181
  • Target name
    ARHGEF2
  • Target description
    Rho/Rac guanine nucleotide exchange factor 2
  • Target synonyms
    GEF; GEFH1; GEF-H1; guanine nucleotide exchange factor H1; Lfc; LFP40; microtubule-regulated Rho-GEF; NEDMHM; P40; proliferating cell nucleolar antigen p40; rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    GEF H1 Antibody (YA1877) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1877), targeting GEF H1, with a predicted molecular weight of 112 kDa (observed band size: 112 kDa). GEF H1 Antibody (YA1877) can be used for WB, ICC/IF, FC experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GEF-H1/ARHGEF2 using anti-GEF-H1/ARHGEF2 antibody (A02572-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GEF-H1/ARHGEF2 antigen affinity purified polyclonal antibody (Catalog # A02572-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GEF-H1/ARHGEF2 at approximately 112 kDa. The expected band size for GEF-H1/ARHGEF2 is at 112 kDa.
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