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Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

GEF-H1 antibody

GTX125893
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARHGEF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    GEF-H1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9181
  • Target name
    ARHGEF2
  • Target description
    Rho/Rac guanine nucleotide exchange factor 2
  • Target synonyms
    GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40, rho guanine nucleotide exchange factor 2, Rho/Rac guanine nucleotide exchange factor (GEF) 2, guanine nucleotide exchange factor H1, microtubule-regulated Rho-GEF, proliferating cell nucleolar antigen p40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92974
  • Protein Name
    Rho guanine nucleotide exchange factor 2
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ueda Y, Higasa K, Kamioka Y, et al. Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1. iScience. 2023,26(8):107292. doi: 10.1016/j.isci.2023.107292
    Read this paper
  • Hetmanski JHR, de Belly H, Busnelli I, et al. Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration. Dev Cell. 2019,51(4):460-475.e10. doi: 10.1016/j.devcel.2019.09.006
    Read this paper
  • Liang Z, Zhan Y, Shen Y, et al. The pseudokinase CaMKv is required for the activity-dependent maintenance of dendritic spines. Nat Commun. 2016,7:13282. doi: 10.1038/ncomms13282
    Read this paper
  • Wang HL, Yang CH, Lee HH, et al. Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction. J Cell Sci. 2015,128(20):3757-68. doi: 10.1242/jcs.169961
    Read this paper
  • Huang IH, Hsiao CT, Wu JC, et al. GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment. J Cell Sci. 2014,127(Pt 19):4186-200. doi: 10.1242/jcs.150227
    Read this paper

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Figure 1. Western blot analysis of GEF-H1/ARHGEF2 using anti-GEF-H1/ARHGEF2 antibody (A02572-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GEF-H1/ARHGEF2 antigen affinity purified polyclonal antibody (Catalog # A02572-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GEF-H1/ARHGEF2 at approximately 112 kDa. The expected band size for GEF-H1/ARHGEF2 is at 112 kDa.
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