More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GEF-H1 antibody
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

GEF-H1 antibody

GTX125893
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARHGEF2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    GEF-H1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9181
  • Target name
    ARHGEF2
  • Target description
    Rho/Rac guanine nucleotide exchange factor 2
  • Target synonyms
    GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40, rho guanine nucleotide exchange factor 2, Rho/Rac guanine nucleotide exchange factor (GEF) 2, guanine nucleotide exchange factor H1, microtubule-regulated Rho-GEF, proliferating cell nucleolar antigen p40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92974
  • Protein Name
    Rho guanine nucleotide exchange factor 2
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HT29 cells using Lfc Polyclonal Antibody
Product group Antibodies
Cusabio
ARHGEF2 AntibodyCSB-PA003148
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice
Anti-ARHGEF2 Antibody
Product group Antibodies
Antibodies.com
Anti-ARHGEF2 AntibodyA100186
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Figure 1. Western blot analysis of GEF-H1/ARHGEF2 using anti-GEF-H1/ARHGEF2 antibody (A02572-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GEF-H1/ARHGEF2 antigen affinity purified polyclonal antibody (Catalog # A02572-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GEF-H1/ARHGEF2 at approximately 112 kDa. The expected band size for GEF-H1/ARHGEF2 is at 112 kDa.
Product group Antibodies
Boster Bio
Anti-GEF-H1/ARHGEF2 Antibody Picoband(r)A02572-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice
Product group Antibodies
MedChem Express
GEF H1 Antibody (YA1877, Rabbit)HY-P82132
TargetARHGEF2
  • SizePrice
Immunohistochemical staining of human bone marrow shows strong positivity in hematopoietic cells.
Product group Antibodies
Atlas Antibodies
Anti-ARHGEF2 AntibodyHPA043437
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetARHGEF2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ARHGEF2 / GEF-H1 AntibodyLS-C404553
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
GEF-H1 antibody [HL3470]GTX641355
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice
Lane 1: 293T Cells; Probed with ARHGEF2 (9D4) Monoclonal Antibody (bsm-54369R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss
GEF H1 Recombinant AntibodyBSM-54369R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice
Product group Antibodies
GeneTex
GEF-H1 antibody [B4/7]GTX54457
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityCanine, Human
TargetARHGEF2
  • SizePrice
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500
Product group Antibodies
GeneTex
GEF-H1 antibody [GT336]GTX629398
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetARHGEF2
  • SizePrice