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ProductsBack/Gephyrin Antibody (clone OTI1C5, Carrier-free)

Gephyrin Antibody (clone OTI1C5, Carrier-free)

LS-C785472
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetGPHN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Gephyrin Antibody (clone OTI1C5, Carrier-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:100), WB (1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C5
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10243
  • Target name
    GPHN
  • Target description
    gephyrin
  • Target synonyms
    GEPH, GPH, GPHRYN, HKPX1, MOCODC, gephyrin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody (A04560-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gephyrin/GPHN antigen affinity purified polyclonal antibody (Catalog # A04560-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gephyrin/GPHN at approximately 93 kDa. The expected band size for Gephyrin/GPHN is at 93 kDa.
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