Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641902/GTX641902_T-45663_20250521_WB_R_brain_25022521_375.webp "Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641902/GTX641902_T-45663_20250521_WB_M_tissue_RPKM_watermark_25022521_201.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
![Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641902/GTX641902_T-45663_20250411_IHC-P_M_25041720_258.webp "Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: GABA B Receptor 1 antibody [GT83] (GTX641938) diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641902/GTX641902_45761_20250822_IHC-P_M_1_25082900_475.webp "Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: GABA B Receptor 1 antibody [GT83] (GTX641938) diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: GABA B Receptor 1 antibody [GT83] (GTX641938) diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641902/GTX641902_45761_20250822_IHC-P_M_2_25082900_503.webp "Gephyrin antibody [HL3712] detects Gephyrin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. Green: Gephyrin stained by Gephyrin antibody [HL3712] (GTX641902) diluted at 1:250. Red: GABA B Receptor 1 antibody [GT83] (GTX641938) diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [HL3712] (GTX641902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Gephyrin antibody [HL3712]
GTX641902
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGPHN
Overview
- SupplierGeneTex
- Product NameGephyrin antibody [HL3712]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3712
- ConjugateUnconjugated
- Gene ID10243
- Target nameGPHN
- Target descriptiongephyrin
- Target synonymsGEPH, GPH, GPHRYN, HKPX1, MOCODC, gephyrin
- HostRabbit
- IsotypeIgG
- Protein IDQ9NQX3
- Protein NameGephyrin
- Scientific DescriptionThis gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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