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ProductsBack/GFI1 Mouse Monoclonal Antibody [Clone ID: 5D7]
Western blot analysis using GFI1 antibody Cat.-No AM06509SU-N against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using GFI1 antibody Cat.-No AM06509SU-N against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using GFI1 antibody Cat.-No AM06509SU-N against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.

GFI1 Mouse Monoclonal Antibody [Clone ID: 5D7]

AM06509SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGFI1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    GFI1 Mouse Monoclonal Antibody [Clone ID: 5D7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D7
  • Gene ID2672
  • Target name
    GFI1
  • Target description
    growth factor independent 1 transcriptional repressor
  • Target synonyms
    GFI-1, GFI1A, SCN2, ZNF163, zinc finger protein Gfi-1, growth factor independence-1, growth factor independent 1 transcription repressor, growth factor independent protein 1, zinc finger protein 163
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99684
  • Protein Name
    Zinc finger protein Gfi-1
  • Scientific Description
    GFI1 mouse monoclonal antibody, clone 5D7, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of GFI1 using anti-GFI1 antibody (A00888-1). GFI1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-GFI1 Antibody (A00888-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph node lysates probed with GFI1/ZNF163 Polyclonal Antibody, Unconjugated (bs-13337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFI1 antibody (GTX133798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TargetGFI1
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