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WB analysis of LOVO cell lysates using GTX87350 GHITM antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of LOVO cell lysates using GTX87350 GHITM antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of LOVO cell lysates using GTX87350 GHITM antibody. The lane on the right is blocked with the synthesized peptide.

GHITM antibody

GTX87350
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetGHITM
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Overview

  • Supplier
    GeneTex
  • Product Name
    GHITM antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27069
  • Target name
    GHITM
  • Target description
    growth hormone inducible transmembrane protein
  • Target synonyms
    DERP2, HSPC282, MICS1, My021, PTD010, TMBIM5, growth hormone-inducible transmembrane protein, dermal papilla-derived protein 2, mitochondrial morphology and cristae structure 1, transmembrane BAX inhibitor motif containing 5, transmembrane BAX inhibitor motif-containing protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3K2
  • Protein Name
    Growth hormone-inducible transmembrane protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GHITM using anti-GHITM antibody (A11256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHITM antigen affinity purified polyclonal antibody (Catalog # A11256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHITM at approximately 37 kDa. The expected band size for GHITM is at 37 kDa.
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