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WB analysis of human cerebellum (A), duodenum (B) and heart (C) cell lysates using GTX88206 GHITM antibody, Internal. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human cerebellum (A), duodenum (B) and heart (C) cell lysates using GTX88206 GHITM antibody, Internal. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human cerebellum (A), duodenum (B) and heart (C) cell lysates using GTX88206 GHITM antibody, Internal. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer

GHITM antibody, Internal

GTX88206
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGHITM
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Overview

  • Supplier
    GeneTex
  • Product Name
    GHITM antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.01-0.1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27069
  • Target name
    GHITM
  • Target description
    growth hormone inducible transmembrane protein
  • Target synonyms
    DERP2, HSPC282, MICS1, My021, PTD010, TMBIM5, growth hormone-inducible transmembrane protein, dermal papilla-derived protein 2, mitochondrial morphology and cristae structure 1, transmembrane BAX inhibitor motif containing 5, transmembrane BAX inhibitor motif-containing protein 5
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9H3K2
  • Protein Name
    Growth hormone-inducible transmembrane protein
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GHITM using anti-GHITM antibody (A11256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHITM antigen affinity purified polyclonal antibody (Catalog # A11256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHITM at approximately 37 kDa. The expected band size for GHITM is at 37 kDa.
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