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ProductsBack/GIT2 Antibody (OAAB18366)

GIT2 Antibody (OAAB18366)

OAAB18366
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetGIT2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GIT2 Antibody (OAAB18366)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID9815
  • Target name
    GIT2
  • Target description
    GIT ArfGAP 2
  • Target synonyms
    ARF GAP GIT2; ARF GTPase-activating protein GIT2; CAT2; CAT-2; cool-associated, tyrosine phosphorylated protein 2; cool-interacting tyrosine-phosphorylated protein 2; G protein-coupled receptor kinase interacting ArfGAP 2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2; Paxillin kinase linker; PKL
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIT2 using anti-GIT2 antibody (A04441-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIT2 antigen affinity purified polyclonal antibody (Catalog # A04441-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIT2 at approximately 85 kDa. The expected band size for GIT2 is at 85 kDa.
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