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ProductsBack/GITR antibody [MAB0860]

GITR antibody [MAB0860]

GTX52441
GeneTex
ApplicationsFlow Cytometry, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetTnfrsf18
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Overview

  • Supplier
    GeneTex
  • Product Name
    GITR antibody [MAB0860]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MAB0860
  • Conjugate
    Unconjugated
  • Gene ID21936
  • Target name
    Tnfrsf18
  • Target description
    tumor necrosis factor receptor superfamily, member 18
  • Target synonyms
    AITR; Gi; Gitr; glucocorticoid-induced TNFR-related protein; tumor necrosis factor receptor superfamily member 18
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDO35714
  • Protein Name
    Tumor necrosis factor receptor superfamily member 18
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody (A03125-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf18 antigen affinity purified polyclonal antibody (Catalog # A03125-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf18 at approximately 30-35KD. The expected band size for Tnfrsf18 is at 26KD.
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