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ProductsBack/GJA1 / CX43 / Connexin 43 Antibody (aa252-270)

GJA1 / CX43 / Connexin 43 Antibody (aa252-270)

LS-C14553
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetGJA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GJA1 / CX43 / Connexin 43 Antibody (aa252-270)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Western Blot (colorimetric detection): 1:1000-1:10000 (Mouse brain lysates). Immunocytochemistry: 1:50-1:250. Immunohistochemistry: 1:50-1:250 (Frozen tissue sections of mouse keratinocytes and rat heart).. ELISA, ICC (1:50 - 1:250), IHC, IHC-Fr, WB (1:1000 - 1:10000) Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Western Blot (colorimetric detection): 1:1000-1:10000 (Mouse brain lysates). Immunocytochemistry: 1:50-1:250. Immunohistochemistry: 1:50-1:250 (Frozen tissue sections of mouse keratinocytes and rat heart).
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2697
  • Target name
    GJA1
  • Target description
    gap junction protein alpha 1
  • Target synonyms
    AVSD3; CMDR; connexin-43; CX43; EKVP; EKVP3; gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa; GJAL; HLHS1; HSS; ODDD; PPKCA
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJA1 using anti-GJA1 antibody (A00599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testicular tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA1 antigen affinity purified polyclonal antibody (Catalog # A00599) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43KD. The expected band size for GJA1 is at 43KD.
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