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WB analysis of Jurkat cell lysates using GTX87800 GJA4 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cell lysates using GTX87800 GJA4 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cell lysates using GTX87800 GJA4 antibody. The lane on the right is blocked with the synthesized peptide.

GJA4 antibody

GTX87800
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetGJA4
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Overview

  • Supplier
    GeneTex
  • Product Name
    GJA4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2701
  • Target name
    GJA4
  • Target description
    gap junction protein alpha 4
  • Target synonyms
    CX37, gap junction alpha-4 protein, connexin-37, gap junction protein, alpha 4, 37kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35212
  • Protein Name
    Gap junction alpha-4 protein
  • Scientific Description
    This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJA4 using anti-GJA4 antibody (A05569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA4 antigen affinity purified polyclonal antibody (Catalog # A05569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA4 at approximately 37 kDa. The expected band size for GJA4 is at 37 kDa.
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