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GKAP1 Polyclonal Antibody

RD218819A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGKAP1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    GKAP1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.62 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80318
  • Target name
    GKAP1
  • Target description
    G kinase anchoring protein 1
  • Target synonyms
    FKSG21, GKAP42, G kinase-anchoring protein 1, cGMP-dependent protein kinase anchoring protein 42kDa, cGMP-dependent protein kinase-anchoring protein of 42 kDa, protein kinase anchoring protein GKAP42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VSY0
  • Protein Name
    G kinase-anchoring protein 1
  • Scientific Description
    This gene encodes a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene. - This is a GKAP1 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GKAP1 using anti-GKAP1 antibody (A14397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GKAP1 antigen affinity purified polyclonal antibody (Catalog # A14397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GKAP1 at approximately 40-42 kDa. The expected band size for GKAP1 is at 42 kDa.
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