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ProductsBack/GLRX2 Antibody - C-terminal region (OAAB04283)

GLRX2 Antibody - C-terminal region (OAAB04283)

OAAB04283
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGLRX2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GLRX2 Antibody - C-terminal region (OAAB04283)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID51022
  • Target name
    GLRX2
  • Target description
    glutaredoxin 2
  • Target synonyms
    CGI-133, GRX2, glutaredoxin 2, bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2), glutaredoxin (thioltransferase) 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of GRX2/GLRX2 using anti-GRX2/GLRX2 antibody (A05331). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRX2/GLRX2 antigen affinity purified polyclonal antibody (Catalog # A05331) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRX2/GLRX2 at approximately 18KD. The expected band size for GRX2/GLRX2 is at 18KD.
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