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GLRX3 / Glutaredoxin 3 Antibody

LS-C748624
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGLRX3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GLRX3 / Glutaredoxin 3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 37kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 37kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10539
  • Target name
    GLRX3
  • Target description
    glutaredoxin 3
  • Target synonyms
    GLRX4, GRX3, GRX4, PICOT, TXNL2, TXNL3, glutaredoxin-3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, PKCq-interacting protein, glutaredoxin 4, testicular tissue protein Li 75, thioredoxin-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: Glutaredoxin-3 antibody at 10ug/ml Lane 1: Rat brain tissue Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
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Figure 1. Western blot analysis of GLRX3 using anti-GLRX3 antibody (A06489). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLRX3 antigen affinity purified polyclonal antibody (Catalog # A06489) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLRX3 at approximately 40 kDa. The expected band size for GLRX3 is at 37 kDa.
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