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TXNL2 antibody [N2C3]

GTX118339
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLRX3
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Overview

  • Supplier
    GeneTex
  • Product Name
    TXNL2 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.61 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10539
  • Target name
    GLRX3
  • Target description
    glutaredoxin 3
  • Target synonyms
    GLRX4; glutaredoxin 4; glutaredoxin-3; GRX3; GRX4; PICOT; PKC-interacting cousin of thioredoxin; PKCq-interacting protein; PKC-theta-interacting protein; testicular tissue protein Li 75; thioredoxin-like protein 2; TXNL2; TXNL3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76003
  • Protein Name
    Glutaredoxin-3
  • Scientific Description
    This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Resources for the Comprehensive Discovery of Functional RNA Elements. Sundararaman B et al., 2016 Mar 17, Mol Cell
    Read more

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Figure 1. Western blot analysis of GLRX3 using anti-GLRX3 antibody (A06489). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLRX3 antigen affinity purified polyclonal antibody (Catalog # A06489) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLRX3 at approximately 40 kDa. The expected band size for GLRX3 is at 37 kDa.
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