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Western blot All lanes: GLS antibody at 5microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74, 18, 66 kDa Observed band size: 74 kDa
Western blot All lanes: GLS antibody at 5microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74, 18, 66 kDa Observed band size: 74 kDa
Western blot All lanes: GLS antibody at 5microg/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74, 18, 66 kDa Observed band size: 74 kDa

GLS Antibody

CSB-PA009528DA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGLS
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Overview

  • Supplier
    Cusabio
  • Product Name
    GLS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2744
  • Target name
    GLS
  • Target description
    glutaminase
  • Target synonyms
    AAD20; CASGID; DEE71; EIEE71; GAC; GAM; GDPAG; GLS1; glutaminase C; glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; KGA; K-glutaminase; L-glutamine amidohydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94925
  • Protein Name
    Glutaminase kidney isoform, mitochondrial
  • Scientific Description
    Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (A01272-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat heart tissue lysates. Lane 8: rat skeletal muscle tissue lysates. Lane 9: mouse brain tissue lysates. Lane 10: mouse kidney tissue lysates. Lane 11: mouse heart tissue lysates. Lane 12: mouse skeletal muscle tissue lysates. Lane 13: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaminase/GLS antigen affinity purified polyclonal antibody (Catalog # A01272-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaminase/GLS at approximately 65-73KD. The expected band size for Glutaminase/GLS is at 73KD.
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