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ProductsBack/Glucokinase (GCK) Mouse Monoclonal Antibody [Clone ID: OTI3E3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCK (Cat# RC200472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK(Cat# TA500459). Positive lysates [LY400059] (100ug) and [LC400059] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCK (Cat# RC200472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK(Cat# TA500459). Positive lysates [LY400059] (100ug) and [LC400059] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCK (Cat# RC200472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK(Cat# TA500459). Positive lysates [LY400059] (100ug) and [LC400059] (20ug) can be purchased separately from OriGene.

Glucokinase (GCK) Mouse Monoclonal Antibody [Clone ID: OTI3E3]

CF500459
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGCK
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Glucokinase (GCK) Mouse Monoclonal Antibody [Clone ID: OTI3E3]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3E3
  • Gene ID2645
  • Target name
    GCK
  • Target description
    glucokinase
  • Target synonyms
    FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, PNDM1, hexokinase-4, ATP:D-hexose 6-phosphotransferase, HK IV, glucokinase (hexokinase 4), hexokinase D, pancreatic isozyme, hexokinase type IV
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35557
  • Protein Name
    Hexokinase-4
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Glucokinase using anti-Glucokinase antibody (A00884-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucokinase antigen affinity purified polyclonal antibody (Catalog # A00884-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glucokinase at approximately 52KD. The expected band size for Glucokinase is at 52KD.
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