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ProductsBack/Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI20E1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.

Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI20E1]

CF500576
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC2A5
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI20E1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI20E1
  • Gene ID6518
  • Target name
    SLC2A5
  • Target description
    solute carrier family 2 member 5
  • Target synonyms
    GLUT-5, GLUT5, solute carrier family 2, facilitated glucose transporter member 5, glucose transporter type 5, small intestine, glucose transporter-like protein 5, solute carrier family 2 (facilitated glucose/fructose transporter), member 5, testicular tissue protein Li 81
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP22732
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 5
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SLC2A5 mouse monoclonal antibody, clone OTI20E1 (formerly 20E1)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PB9960) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A5 at approximately 55KD. The expected band size for SLC2A5 is at 55KD.
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