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Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (GTX638096) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (GTX638096) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (GTX638096) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GLUD1+ GLUD2 antibody [HL2124]

GTX638096
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGLUD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GLUD1+ GLUD2 antibody [HL2124]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2124
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2746
  • Target name
    GLUD1
  • Target description
    glutamate dehydrogenase 1
  • Target synonyms
    GDH, GDH1, GLUD, hGDH1, glutamate dehydrogenase 1, mitochondrial, epididymis secretory sperm binding protein, epididymis tissue sperm binding protein Li 18mP, glutamate dehydrogenase (NAD(P)+)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00367
  • Protein Name
    Glutamate dehydrogenase 1, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUD1/2 antigen affinity purified polyclonal antibody (Catalog # A01866-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUD1/2 at approximately 54 kDa. The expected band size for GLUD1/2 is at 61 kDa.
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