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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-GLUR3 Polyclonal Antibody, Unconjugated (bs-1799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-GLUR3 Polyclonal Antibody, Unconjugated (bs-1799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-GLUR3 Polyclonal Antibody, Unconjugated (bs-1799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n

Glutamate receptor 3 Polyclonal Antibody

BS-1799R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGRIA3
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Overview

  • Supplier
    Bioss
  • Product Name
    Glutamate receptor 3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2892
  • Target name
    GRIA3
  • Target description
    glutamate ionotropic receptor AMPA type subunit 3
  • Target synonyms
    GLUR-C, GLUR-K3, GLUR3, GLURC, GluA3, MRX94, MRXSW, iGluR3, glutamate receptor 3, AMPA receptor subunit GluA3, AMPA-selective glutamate receptor 3, dJ1171F9.1, gluR-3, glutamate receptor C, glutamate receptor subunit 3, glutamate receptor, ionotrophic, AMPA 3, glutamate receptor, ionotropic, AMPA 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42263
  • Protein Name
    Glutamate receptor 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GRIA3 using anti-GRIA3 antibody (PB9206). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA3 antigen affinity purified polyclonal antibody (Catalog # PB9206) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA3 at approximately 101 kDa. The expected band size for GRIA3 is at 101 kDa.
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